Sorting nexin 12 interacts with BACE1 and regulates BACE1-mediated APP processing
© Zhao et al.; licensee BioMed Central Ltd. 2012
Received: 16 March 2012
Accepted: 24 May 2012
Published: 18 June 2012
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© Zhao et al.; licensee BioMed Central Ltd. 2012
Received: 16 March 2012
Accepted: 24 May 2012
Published: 18 June 2012
β-site APP cleaving enzyme 1 (BACE1) cleaves β-amyloid precursor protein (APP) to initiate the production of β-amyloid (Aβ), the prime culprit in Alzheimer’s disease (AD). Dysregulation of the intracellular trafficking of BACE1 may affect Aβ generation, contributing to AD pathology. In this study, we investigated whether BACE1 trafficking and BACE1-mediated APP processing/Aβ generation are affected by sorting nexin 12 (SNX12), a member of the sorting nexin (SNX) family that is involved in protein trafficking regulation.
Herein, we find that SNX12 is widely expressed in brain tissues and is mainly localized in the early endosomes. Overexpression of SNX12 does not affect the steady-state levels of APP, BACE1 or γ-secretase components, but dramatically reduces the levels of Aβ, soluble APPβ and APP β-carboxyl terminal fragments. Downregulation of SNX12 has the opposite effects. Modulation of SNX12 levels does not affect γ-secretase activity or in vitro β-secretase activity. Further studies reveal that SNX12 interacts with BACE1 and downregulation of SNX12 accelerates BACE1 endocytosis and decreases steady-state level of cell surface BACE1. Finally, we find that the SNX12 protein level is dramatically decreased in the brain of AD patients as compared to that of controls.
This study demonstrates that SNX12 can regulate the endocytosis of BACE1 through their interaction, thereby affecting β-processing of APP for Aβ production. The reduced level of SNX12 in AD brains suggests that an alteration of SNX12 may contribute to AD pathology. Therefore, inhibition of BACE1-mediated β-processing of APP by regulating SNX12 might serve as an alternative strategy in developing an AD intervention.
A major pathological hallmark of Alzheimer’s disease (AD) is the formation of senile plaques in the brain. The major components of senile plaques are heterogeneous small peptides called β-amyloid (Aβ) . Aβ peptides are derived from the β-amyloid precursor protein (APP) through sequential proteolytic cleavages; first by β-secretase and then by γ-secretase. β-cleavage of APP generates a soluble APPβ (sAPPβ) fragment and a membrane-associated APP β-carboxyl terminal fragment (βCTF). The latter can then be cleaved by γ-secretase to release Aβ. γ-secretase is a high molecular weight complex that consists of presenilin 1 (PS1) or presenilin 2 (PS2), nicastrin, APH1 and PEN-2. Alternatively, APP can be cleaved by α-secretase within the Aβ sequence to release soluble APPα (sAPPα), which is neuroprotective, precluding Aβ generation . Accumulating evidence has shown that overproduction/accumulation of Aβ peptides in vulnerable brain regions is a primary cause in the pathogenesis of AD [2–4]. Therefore, a dysregulation of the proteins involved in Aβ generation, including the altered intracellular trafficking/subcellular localization of them, may increase Aβ generation and lead to disease pathogenesis.
The type I transmembrane aspartyl protease, β-site APP cleaving enzyme 1 (BACE1), is the putative β-secretase [5–8]. Because its optimal activity requires an acidic environment, BACE1 is mainly localized in the early Golgi, late Golgi/early endosomes, and endosomes. In addition, BACE1 can be found at the cell surface [6, 9–12]. Although several proteins have been found to interact with BACE1 and regulate its intracellular trafficking, such as reticulon/Nogo [13–15], Golgi-localized γ-ear-containing ARF-binding (GGA) [16–18] and sorting nexin 6 (SNX6) , the detailed mechanism underlying BACE1 trafficking regulation has yet to be fully elucidated.
Sorting nexins (SNXs) are a diverse group of cellular trafficking proteins that are unified by the presence of a phospholipid-binding (PX) motif. The ability of SNXs to bind specific phospholipids, as well as their propensity to form protein-protein complexes, suggests a role for these proteins in membrane trafficking and protein sorting [20–22]. Recently, some SNX members have been found to mediate APP processing/Aβ generation through regulating trafficking of AD associated proteins: SNX17 can interact with APP in the early endosome and downregulation of SNX17 leads to reduced steady-state levels of APP with a concomitant increase in Aβ production . SNX33 can bind to the endocytic GTPase dynamin and overexpression of SNX33 reduces the rate of APP endocytosis in a dynamin-dependent manner, so that APP is subjected to increased α-cleavage in cell surface . Moreover, SNX6 can associate with BACE1 and a reduction of SNX6 results in elevated steady-state levels of BACE1 as well as increased retrograde transport of BACE1 in the endocytic pathway, increasing Aβ generation . However, the functional roles of other SNX proteins, especially their involvement in AD, deserve further scrutiny.
In the present study, we demonstrate that another SNX member, SNX12, can interact with BACE1 and affects its intracellular trafficking, thus mediating β-processing of APP in Aβ production.
Aβ is generated from its precursor APP through sequential cleavages first by β-secretase/BACE1 and then by γ-secretase, during their highly regulated trafficking within the cell. Dysregulation in the intracellular trafficking of these proteins may alter a proper interaction between APP and its two secretases, thereby affecting Aβ production and contributing to disease pathogenesis. The putative β-secretase, BACE1, cycles between the secretory pathway compartments while BACE1 activity resides in both the secretory pathway and the endosomes. The intracellular trafficking of BACE1 may be affected by molecules that interact with it: some studies found that BACE1 can interact with reticulon/Nogo proteins, whose increased expression can block BACE1 in the ER that has a neutral pH environment and thus inhibit BACE1 activity in Aβ generation [13–15]. In addition, Golgi-localized γ-ear-containing ARF-binding (GGA) proteins interact with BACE1 and regulate its trafficking between the late Golgi and early endosomes; depletion of GGA proteins increases the accumulation of BACE1 in the acidic early endosome for enhanced BACE1 stability and cleavage of APP [16–18]. More recently, a SNX family member, SNX6, has also been found to be associated with BACE1 and a reduction of SNX6 results in elevated steady-state levels of BACE1 as well as increased retrograde transport of BACE1 in the endocytic pathway, causing increased Aβ generation .
In this study, we demonstrate that SNX12, another SNX family member, also interacts with BACE1 and affects the β-cleavage of APP. Although the steady-state level of BACE1 was not affected upon modulation of SNX12, downregulation of SNX12 resulted in increased BACE1 endocytosis and Aβ generation, similar to that of SNX6; and overexpression of SNX12 reduced the production of Aβ. Importantly, we found that SNX12 was abundantly expressed in brain tissues and the level of SNX12 was dramatically decreased in the brain of AD patients when compared to that of controls, suggesting a direct link between SNX12 alteration and AD pathology.
SNX family members share a conserved PX motif that acts as a phosphoinositide-binding site for targeting the SNX protein to phosphoinositide-enriched membranes . These proteins have been proposed to play a role in membrane trafficking and protein sorting [20–22]. In addition to SNX12 and SNX6 which interact with BACE1 and affect APP processing/Aβ generation, several other SNX members such as SNX17  and SNX33  have also been found to mediate APP processing/Aβ generation through regulating the intracellular trafficking of APP. Together, these results suggest that SNX members may participate in AD pathology through modulating the trafficking of various AD-associated proteins, which deserves further investigation.
Our studies have demonstrated that SNX12 may participate in AD pathology by interacting with BACE1, thereby regulating BACE1 intracellular trafficking and BACE1-mediated β-processing of APP in Aβ production. The reduction of SNX12 in the brain of AD patients may accelerate the endocytosis of BACE1 and lead to increased Aβ generation. As BACE1 represents a favored target for AD intervention, inhibition of BACE1-mediated β-processing of APP by regulating SNX12 might provide an alternative strategy for developing AD therapeutics.
HEK293T and SH-SY5Y cells were maintained in DMEM (Hyclone) supplemented with 10% FBS (Hyclone) and 1% penicillin/streptomycin. HEK cells stably expressing human APP Swedish mutations (HEK-APPSwe) were maintained in the same media with the addition of 400 μg/mL G418 (Invitrogen). Hela cells were maintained in RPMI-1640 (Hyclone) supplemented with 10% FBS and 1% penicillin/streptomycin. Maintenance of N2a cells stably expressing human APP695 (N2a-APP) was as previously reported . Primary neurons derived from APP/PS1/tau triple transgenic mice collected at postnatal day 0 were cultured in Neurobasal medium supplemented with B-27 (Invitrogen).
The pCI-neo-SNX12/myc plasmid and the pCI-neo control plasmid were kindly provided by Dr. Wanjin Hong. The BACE1-HA plasmid was kindly provided by Dr. Riqiang Yan. The APP βCTF (containing the last 99 carboxyl-amino acids, C99) and ΔC57 (lacking the last 57 carboxyl-terminal amino acids) fragments were inserted into the pcDNA3.1-Myc/His plasmid (Invitrogen) to generate plasmids. The SNX12-EGFP plasmid was constructed by subcloning SNX12 into the pEGFP-N1 plasmid (Clontech). Notch NΔE plasmid was as previously reported [27, 30].
Transient transfection was performed by using TurboFect reagent (Fermentas), following the manufacturer’s protocol.
Antibodies used in this study include: anti-Myc antibody (9E10) from Santa Cruz Biotechnology; anti-HA and anti-SNX12 antibodies from Gene Tex; anti-α-tubulin and anti-β-actin antibodies and control IgG from Sigma; anti-Aβ antibody (6E10) from Covance; and anti-EEA1 and anti-cd63 antibodies from Abcam. Anti-BACE1 antibody 689 and anti-APP antibody 369 were kindly provided by Dr. Huaxi Xu. Anti-BACE1 antibody 3D5 was kindly provided by Dr. Robert Vassar.
Cells were lysed in a lysis buffer containing 10 mM Tris–HCl (pH 7.8), 1 mM EDTA, 150 mM NaCl, 1% Nonidet P-40 and supplemented with a protease inhibitor mixture. Equal protein amounts of cell lysates were subjected to SDS-PAGE and Western blot with indicated antibodies.
For RNA interference (RNAi) to downregulate human SNX12 expression, two SNX12 targeting siRNAs (1: 5’- ATGAGCTGGAGAGAGATAG-3’; and 2: 5’- TGAACGCTGCCTACACATG-3’) and a scrambled control siRNA (Invitrogen) were transfected into cells using Lipofectamine RNAiMAX reagent (Invitrogen), following the manufacturer’s protocol.
SNX12 shRNA and control lentiviral particles were purchased from Santa Cruz Biotechnology. These viruses were used to infect APP/PS1/tau primary neurons for 72 h before experiments.
Cells were lysed in a lysis buffer. Cell lysates were pre-cleared with rProtein A sepharose (GE) at 4 °C for 1 h and then incubated with rProtein A sepharose and the indicated antibodies or IgG at 4 °C overnight. Immunoprecipitated proteins were analyzed by Western blot.
To detect the total level of secreted Aβ, cells were transfected for 24-48 h and then incubated with DMEM for 4-6 h. Conditioned media was then collected and incubated with trichloroacetic acid (1:9 v/v) overnight at 4 °C for protein precipitation. Precipitated proteins were subjected to Western blot with the anti-Aβ antibody 6E10. Alternatively, Conditioned media were assayed by ELISA to detect Aβ40 and Aβ42, using commercial ELISA kits (Invitrogen).
The in vitro BACE1 activity was assayed by using the β-Secretase Activity Assay Kit, Fluorogenic (Calbiochem), following the manufacturer’s protocol.
Treated cells were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton-X100. Cells were then incubated with the indicated primary antibodies, followed by incubation with Alexa 594-conjugated secondary antibody (Invitrogen). Specimens were examined with a ZEISS LSM 5 Exciter confocal microscope and images were captured using the ZEN software.
Treated cells were washed with ice-cold phosphate-buffered saline containing 1 mM each of CaCl2 and MgCl2, and incubated at 4 °C with 0.5 mg/mL Sulfo-NHS-LC-biotin (Thermo Scientific, Rockford, IL). Cells were then lysed and lysates were affinity-precipitated with streptavidin-agarose beads (Thermo Scientific). Biotinylated proteins were subjected to Western blot analysis.
Cells were first biotinylated with cleavable EZ-Link Sulfo-SS-Biotin (Thermo Scientific). After chasing at 37 °C for the indicated times, cells were treated with cold glutathione buffer at 4 °C to cleave biotin from cell surface proteins. The remaining biotinylated proteins represent the endocytosed proteins and were affinity-precipitated with streptavidin-agarose beads for further Western blot analysis.
Human brain samples used in this study
Two-tailed paired t-test was carried out for statistical analysis.
β-amyloid precursor protein
β-site APP cleaving enzyme 1
Carboxyl terminal fragment
Sorting nexin 12.
We thank Drs. Yong Shen for human brain specimen, Wanjin Hong for SNX12 and control plasmids, Riqiang Yan for BACE1 plasmid, Huaxi Xu for the 689 and 369 antibodies, and Robert Vassar for the 3D5 antibody. This study was supported by grants from the Alzheimer’s Association, National Natural Science Foundation of China (30973150, 81161120496 and 81000540), 973 Prophase Project (2010CB535004), Natural Science Foundation of Fujian Province of China (2009J06022 and 2010J01235), the Program for New Century Excellent Talents in Universities (NCET), the Fundamental Research Funds for the Central Universities, and Fok Ying Tung Education Foundation.
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