Antigen/species | Antibody name /catalog number | Epitope | Concentration immunoblotting | Concentration IHC or IF | Source |
---|---|---|---|---|---|
Primary antibodies | |||||
α-syn/ mouse | Syn1 / 610,787 | 15–123 | 1:1000 | 1:1000 | BD Laboratory (Billerica, MA, USA) |
Human α-syn/ mouse | LB509 / 180,215 | 115–122 | 1:500 | ThermoFisher Scientific (Waltham, MA, USA) | |
α-syn/ rabbit | Syn FL-140 / sc-10,717 | 65–91 | 1:1000 | Santa Cruz Biotech (Dallas, TX, USA) | |
α-syn/ rabbit | Anti-Alpha-synuclein antibody [MJFR1]/ (ab138501) | 118–123 | 1:1000 | Abcam (Cambridge, UK) | |
pS129 α-Syn/ mouse | WAKO / pSyn #64 | Phospho-Ser129 | 1:1000 | 1:2000 | WAKO (Richmond, VA, USA) |
pS129 α-Syn/rabbit | Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y]/ Ab51253 | Phospho-Ser129 | 1:1000 | Abcam (Cambridge, UK) | |
pS129 α-Syn/mouse | pS129 (Ghanem et al., 2022) | Phospho-Ser129 | 100 ng/ml | Ghanem et al., 2022 | |
Beta-actin/ mouse | β-actin clone BA3R / G043 | Beta-actin N-terminal peptide-KLH conjugates. | 1:10000 | Abm (Vancouver, BC, Canada) | |
Drebrin/mouse | Drebrin clone MX823/612,128 | C-terminal 632–649 coupled to KLH | 1:1000 | Progen (Heidelberg, Germany) | |
GAPDH/ mouse | GAPDH loading control/G041 | – | 1:2500 | Abm (Vancouver, BC, Canada) | |
GFP/ rabbit | GFP/A-6455 | Full length protein | 1:5000 | ThermoFisher Scientific (Waltham, MA, USA) | |
Non-phosphorylated α-syn/ mouse | 4B1 | 125–133 | 100 ng/ml | Ghanem et al., 2022 | |
Full-length α-syn aggregates /mouse | Syn-O2 | 100 ng/ml | Ghanem et al., 2022 | ||
Full-length α-syn aggregates /mouse | Syn-O1 | 100 ng/ml | Ghanem et al., 2022 | ||
Full-length α-syn aggregates /mouse | Syn-O3 | 100 ng/ml | Ghanem et al., 2022 | ||
Full-length α-syn aggregates /mouse | Syn-F1 | 100 ng/ml | Ghanem et al., 2022 | ||
Full-length α-syn aggregates /mouse | Syn-F2 | 100 ng/ml | Ghanem et al., 2022 | ||
GFAP/ rabbit | anti-Glial Fibrillary Acidic Protein / Z0334 | Full length protein | 1:800 | Dako (Santa Clara, CA, USA) | |
GFAP/ mouse | Anti-Glial Fibrillary Acidic Protein clone GA5/ MAB360 | Full length protein | 1:800 | Millipore (Temecula, CA, USA) | |
Iba1/ rabbit | Anti-Ionized calcium binding adaptor molecule 1/ 019-19741 | Synthetic peptide (Iba1 C-terminal sequence) | 1:750 | WAKO (Richmond, VA, USA) | |
mCherry/ rabbit | Anti-mCherry antibody/ AB167453 | mCherry | 1:1000 | 1:1000 | Abcam (Cambridge, UK) |
NeuN/ mouse | Anti-NeuN Antibody, clone A60 / MAB377 | 1:1000 | Millipore (Temecula, CA, USA) | ||
PSD95/mouse | PSD95 clone K28/43/75–028 | 77–299 (PDZ domains 1 and 2) | 1:1000 UC | Davis/NIH NeuroMab Facility (Davis, CA, USA) | |
TH/ mouse | Anti-Tyrosine Hydroxylase clone LNC1 /MAB318 | Recognizes an epitope on the outside of the regulatory N-terminus | 1:1000 | Millipore (Temecula, CA, USA) | |
Synpato-physin/ rabbit | Synaptophysin/PA1–1043 | 253–272 1:50000– | 1:25000 | Invitrogen (Waltham, MA, USA) | |
Secondary antibodies | |||||
IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody | 680RD-conjugated goat anti-rabbit/ 926–68,071 | 1:20000 | LI-COR Biosciences (Lincoln, NE, USA) | ||
IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody | 800CW-conjugated goat anti-rabbit/ 926–32,211 | 1:20000 | LI-COR Biosciences (Lincoln, NE, USA) | ||
IRDye 680RD Goat anti-Mouse IgG Secondary Antibody | 680RD-conjugated goat anti-mouse/ 926–68,070 | 1:20000 | LI-COR Biosciences (Lincoln, NE, USA) | ||
IRDye 800CW Goat anti-Mouse IgG Secondary Antibody | 800CW-conjugated goat anti-mouse/ 926–32,210 | 1:20000 | LI-COR Biosciences (Lincoln, NE, USA) | ||
Goat Anti-Mouse IgG Antibody (H + L), Biotinylated | Biotinylated Goat Anti-mouse/ BA-9200 | 1:500 | Vector Laboratories (Burlingame, CA, USA) | ||
Alexa Fluor 488 goat anti-rabbit (H + L) | Alexa Fluor 488 goat anti-rabbit/ A-11,008 | 1:1000 | Invitrogen (Waltham, MA, USA) | ||
Alexa Fluor 633 goat anti-rabbit (H + L) | Alexa Fluor 633 goat anti-rabbit/ A21071 | 1:1000 | Invitrogen (Waltham, MA, USA) | ||
Alexa Fluor 488 goat anti-mouse (H + L) | Alexa Fluor 488 goat anti-mouse/ A-11,029 | 1:1000 | Invitrogen (Waltham, MA, USA) | ||
Alexa Fluor 633 goat anti-mouse (H + L) | Alexa Fluor 633 goat anti-mouse/ A-21,052 | 1:1000 | Invitrogen (Waltham, MA, USA) | ||
Cy™3 AffiniPure Goat Anti-Rabbit IgG (H + L) | 111-165-003 | 1:1000 | Jackson ImmunoResearch Laboratories, Inc 872 West Baltimore Pike West Grove, Pennsylvania 19,390 USA |