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Table 1 FLIM assay detects SPP dimer formation of fusion proteins.

From: Signal peptide peptidase (SPP) dimer formation as assessed by fluorescence lifetime imaging microscopy (FLIM) in intact cells

Donor (CFP) Acceptor (YFP) CFP lifetime (mean ± st.err, psec) P value (Compared to CFP donor only)
NH2-terminus (n = 26) empty vector 2267 ± 60 -
NH2-terminus (n = 22) NH2-terminus 1757 ± 125 < 0.001
COOH-terminus (n = 20) COOH-terminus 1494 ± 145 < 0.001
NH2-terminus (n = 6) COOH-terminus 1526 ± 210 < 0.001
  1. SPP dimer formation between all combinations of fusion protein was demonstrated by significant shortening of donor lifetime. CHO cells co-transfected with different combinations of NH2- or COOH-labeled SPP fusion protein constructs. All CFP-SPP donor lifetimes were significantly shortened in the presence of another YFP-SPP molecule. ANOVA was performed using a Dunnet's post-hoc t-test.