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Table 1 FLIM assay detects SPP dimer formation of fusion proteins.

From: Signal peptide peptidase (SPP) dimer formation as assessed by fluorescence lifetime imaging microscopy (FLIM) in intact cells

Donor (CFP)

Acceptor (YFP)

CFP lifetime (mean ± st.err, psec)

P value (Compared to CFP donor only)

NH2-terminus (n = 26)

empty vector

2267 ± 60

-

NH2-terminus (n = 22)

NH2-terminus

1757 ± 125

< 0.001

COOH-terminus (n = 20)

COOH-terminus

1494 ± 145

< 0.001

NH2-terminus (n = 6)

COOH-terminus

1526 ± 210

< 0.001

  1. SPP dimer formation between all combinations of fusion protein was demonstrated by significant shortening of donor lifetime. CHO cells co-transfected with different combinations of NH2- or COOH-labeled SPP fusion protein constructs. All CFP-SPP donor lifetimes were significantly shortened in the presence of another YFP-SPP molecule. ANOVA was performed using a Dunnet's post-hoc t-test.
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Molecular Neurodegeneration

ISSN: 1750-1326