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Figure 2 | Molecular Neurodegeneration

Figure 2

From: Tumor suppressor PTEN affects tau phosphorylation: deficiency in the phosphatase activity of PTEN increases aggregation of an FTDP-17 mutant Tau

Figure 2

PTEN affects cellular distribution and binding to microtubule of the FTDP-17 mutant tau. A. The fractionation scheme used to resolve different cellular pools of tau. Total membrane and cytosolic fractions were prepared. The tau fraction sedimenting with total membranes (Pellet 1) was treated with nocodazole to solubilize microtubule-associated tau (microtubule-bound tau). The remaining membrane-bound/aggregated tau (Pellet 2) was treated with Na2CO3 to extract membrane-associated tau (membrane-bound tau). The remaining tau aggregates (Pellet 3) were dissolved by SDS. B. Western blot analysis of expression of PTEN and of tau in various celluar fractions. C. Quantification analysis. Data represent mean ± SE, n = 3, *p < 0.02.

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Molecular Neurodegeneration

ISSN: 1750-1326