PTEN affects aggregation of the mutant tau. A. COS-7 cells transiently overexpressing the FTDP-17 mutant tau were transfected with PTEN-WT, PTEN-CG or control vectors. SDS-insoluble aggregated tau proteins were detected by using a filter-trap/immunoblotting assay. The transfected PTEN and total tau proteins (as indicated in middle and lower panels) were detected by Western blots from same amounts of lysates as in filter assays. B. The amounts of aggregated tau were quantified by densitometry and normalized against the amount of total tau. Error bars indicate means ± SE, n = 3, *p < 0.02 compared to the vector transfection control.