Figure 1
From: Aβ reduction in BACE1 heterozygous null 5XFAD mice is associated with transgenic APP level
BACE1 levels are reduced by ~50% in 5XFAD/BACE1 +/− mice. (A) 20 μg of protein/lane of brain homogenates of 4, 6, and 9 month-old 5XFAD/BACE1+/+, 5XFAD/BACE1+/− or 5XFAD/BACE1−/− (as a negative control) female (F) and male (M) mice were separated by SDS-PAGE, transferred onto PVDF membrane, stained with Ponceau S, and incubated with BACE-Cat1 monospecific antibody against BACE1 [28] followed by HRP-conjugated secondary antibody and chemiluminescence imaging. Representative BACE1 immunoblot and Ponceau S staining of brain homogenates from 4 month-old mice is shown. Note the absence of BACE1 immunosignal in the BACE1−/− lane. (B-D) Relative quantification of BACE1 levels in brain homogenates of 4, 6, and 9 month-old female and male 5XFAD/BACE1+/+, 5XFAD/BACE1+/− or 5XFAD/BACE1−/− mice. BACE1 immunosignals were normalized to Ponceau S staining intensity per lane, averaged for each group, then presented as percentage of mean female 5XFAD/BACE1+/+ BACE1 level. At all ages, BACE1 level is significantly reduced by ~50% in 5XFAD/BACE1+/− mice compared to 5XFAD/BACE1+/+ mice, and there is no difference in BACE1 level between male and female 5XFAD/BACE1+/+ or male and female 5XFAD/BACE1+/− mice.