Figure 5
From: Absence of dopaminergic neuronal degeneration and oxidative damage in aged DJ-1-deficient mice
Normal morphology of noradrenergic neuron and no noradrenergic neuron loss in LC in aged DJ-1 -/- mice. (A and B) A substantial level of expression of DJ-1 in LC in control mice is indicated by the presence of DJ-1 immunoreactivity in the LC of wild-type controls and the absence of the immunoreactivity in DJ-1-/- mice. Any immunoreactivity in DJ-1-/- mice indicates non-specific staining. (C and D) Similar TH staining in the LC of DJ-1-/- mice and wild-type controls showing normal morphology of noradrenergic neurons in LC in aged DJ-1-/- mice at the age of 24–27 months. (E and F) Similar 4HNE staining in the LC of DJ-1-/- mice and wild-type controls showing no abnormal accumulation of lipid peroxidation products in aged DJ-1-/- mice. Insets in panels indicate enlarged view of each section. Scale bars; A-F, 0.02 mm; insets, 0.02 mm. (G) The total numbers of TH-positive LC neurons of both sides in all sections from rostral to caudal showing similar numbers of TH-positive neurons in the LC of DJ-1-/- mice and wild-type controls at the age of 24–27 months (+/+: 1145 ± 87, -/-: 1073 ± 151, p > 0.05) (n = 8 per genotype). All data are expressed as mean ± SEM.