ApoER2 increases full length APP association to lipid rafts in LRP1-null CHO cells. (A) Increased association of APP to lipid rafts in ApoER2-expressing cells. LRP1-null CHO cells were transiently transfected with pcDNA3 (control) and APP695-Myc (APP) or APP695-Myc along with ApoER2-HA, treated with 10 μM DAPT for 16 h and lipid rafts were isolated in Lubrol 0.5% – containing lysis buffer in a sucrose gradient. After concentration of sucrose gradients fractions, proteins were subjected to 6% SDS-PAGE, blotted and probed with anti-APP and anti-ApoER2 antibodies. To demonstrate that ApoER2 expression does not disrupts lipid rafts formation in APP expressing cells, 1/16 sucrose gradients fractions were subjected to 12.5% SDS-PAGE and probed with anti-caveolin antibody. ApoER2 increases lipid raft association of full length APP. (B), densitometric analysis of a representative Western blot experiment of (A).