Functional screen for regulators of APP metabolism. (A) Model depicting APP-Gal4 reporter system. (B) Firefly luciferase activity is significantly increased in SH-SY5Y cells stably expressing APP-Gal4 and Gal4-UAS Luciferase compared to SY5Y cells stably expressing either Gal4/Gal4-UAS Luciferase or APP*-Gal4/Gal4-UAS Luciferase. Luciferase activity was normalized to total cell number using SYBR Green. Bars represent mean normalized luciferase expression (+/- std. error) of 16 independent trials for each cell line. Student's t-tests with sequential Bonferroni correction for multiple comparisons; ** indicates p < 0.01.