Figure 5
From: An AICD-based functional screen to identify APP metabolism regulators
Knock-down of APP and individual secretase genes in SY5Y-APP-GAL4 cells decreases AICD-Gal4 mediated luciferase activity. (A) APP-specific shRNA decreases full-length APP, C83-Gal4, and AICD-Gal4 levels compared to the control or "non-silencing" shRNA. Results from duplicate transfections with each shRNA are shown. (B) ADAM10 specific shRNAs decrease endogenous ADAM10, C83-Gal4, and AICD-Gal4 levels compared to the control shRNA. Results from duplicate transfections with each shRNA are shown. (C) ADAM17 specific shRNAs decrease endogenous ADAM17, C83-Gal4, and AICD-Gal4 levels compared to the control shRNA. Results from duplicate transfections with each shRNA are shown. (D) Knock-down of ADAM9, 10, and 17 decrease sAPPα levels compared to control shRNA. (E) Quantification of Western blot densitometries in panels B – D. (F) Transfection with shRNAs specific for APP and individual secretase genes decreases AICD-Gal4-mediated luciferase expression compared to control shRNA. Bars represent the mean normalized luciferase activity of four independent trials and error bars represent standard errors. Statistical significance was determined using two-sample, one tailed t-tests to compare each secretase shRNA with the control shRNA and sequential Bonferroni procedure to adjust for multiple comparisons. * indicates p < 0.05; ** indicates p < 0.01.