Genetic alteration of Fe65 or Tip60 levels modulates AICD-Gal4 mediated luciferase activity. (A) Transient over-expression of Tip60 or Fe65 in SY5Y-APP-Gal4 cells increases AICD-Gal4 production compared to empty vector controls. (B) Knock-down of Fe65 or Tip60 in SY5Y-APP-Gal4 cells decreases AICD-Gal4 mediated luciferase activity Bars represent the mean normalized luciferase activity of four independent trials and error bars represent standard errors. Statistical significance was determined using two-sample, one-tailed t-tests to compare each secretase gene and "vector" or "control" and sequential Bonferroni procedure to adjust for multiple comparisons. * indicates p < 0.05.