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Table 1 Size of DNA fragments obtained by PCR with the novel oligonucleotides tested in this study.

From: Design of an improved set of oligonucleotide primers for genotyping MeCP2tm1.1BirdKO mice by PCR

 

P3 RV

GF RV

 

CCACCCTCCAGTTTGGTTTA

GTTTTGTTCCCCACCCTCCA

GF KO FW

475

486

ACTTTGTCCTGCTGCCTCCA

  

P3 KO FW

458

469

CCATGCGATAAGCTTGATGA

  

P3 WT FW

411

421

GACCCCTTGGGACTGAAGTT

  

GF WT FW

413

424

TCGGACCCCTTGGGACTGA

  
  1. These oligonucleotides were chosen by submitting sequences AM691835 and AM691836 to the P3 [8] and gene Fisher [9] primer-design web servers.
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Molecular Neurodegeneration

ISSN: 1750-1326