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Figure 7 | Molecular Neurodegeneration

Figure 7

From: Fibril specific, conformation dependent antibodies recognize a generic epitope common to amyloid fibrils and fibrillar oligomers that is absent in prefibrillar oligomers

Figure 7

Western blot analysis of Triton X-100 insoluble fractions from human AD brain lysates. Frontal cortex samples, either untreated or formic acid treated, from 6 individual AD brains were dissolved in SDS sample buffer and electrophoresed on polyacrylamide gels and probed with OC or 4G8 as a control. Upper panels: For untreated samples, both OC and 4G8 detect high molecular weight material that accumulates at the top of the gel. Bottom panels: After formic acid treatment, 4G8 detects some lower MW bands in addition to formic acid resistant high MW material, while the staining by OC is abolished, indicating that the staining is conformation dependent.

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