Figure 5

In the neuroregeneration study, it is possible that the observed neuroregenerative effect from intrastriatal grafting with NTN-c17.2 and Mock-c17.2 cells may be due to partial differentiation of cells into TH-positive cells and the expression of neurotrophic factors by NSCs in vivo. A-I, Double immunohistochemistry of TH (B, E, H), LacZ (A, D), and NTN(G) revealed that some grafted c17.2, NTN-c17.2, and Mock-c17.2 cells could differentiate into TH-positive cells 30 d postgrafting. J, Nonquantitative RT-PCR of neurotrophic factor genes using mouse-specific primers (mouse-specific) demonstrated the expression of these genes in Mock-c17.2 (lane 1), NTN-c17.2 cells (lane 4), rat striatum grafted with Mock-c17.2 cells (lane 2), and NTN-c17.2 cells (lane 5) (30 d after grafting), but this expression was not observed after RT-PCR of the cDNA of control rat cerebrum (lane 3). The endogenous control β-actin was amplified using mouse-specific primers in lanes 1, 2, 4, and 5 with rat-specific primers in lane 3.