Figure 5
T0 down-regulates LPS- and Aβ – induced iNOS synthesis and NO production in primary rat microglia. Rat microglia was pre-treated with increasing concentration of T0 (LPS+T0) or vehicle (LPS only) prior to the addition of LPS (100 ng/ml) and the ligand was co-applied with LPS. Control cells received vehicle only (veh). Cells were harvested 24 hours after LPS administration and the expression of iNOS was examined by WB. A and B: Concentration dependent effect of T0 on LPS-induced iNOS protein and NO production in primary rat microglia. Microglia was pre-treated with increasing concentration of T0 (LPS+T0) or vehicle (LPS only) prior to the addition of LPS (100 ng/ml) and the ligand was co-applied with LPS. Control cells received vehicle only (veh). Cells were harvested 24 after LPS treatment and the expression of iNOS was examined by WB (A). NO in the conditioned media was measured by Griess reagent (B). C and D: T0 (10 μM) inhibits NO production (C) and iNOS protein (D) induced by fibrillar Aβ25–35 or Aβ42 peptides applied for 24 hours to microglia. Note that T0 decreases iNOS even bellow its basal level if applied with Aβ (in D, veh versus Aβ25–35+T0, p < 0.01). Values are means ± SEM; two-tailed Student's t test; *, p < 0.05.