Figure 6
LXR ligands decrease the secretion of pro-inflammatory cytokines in primary rat microglial cells treated with LPS or fibrillar Aβ 25–35 . A: Rat microglia were treated with increasing concentrations of T0 and LPS as in Fig. 5 and IL1-β secretion measured by ELISA. B and C: Rat microglia were treated with 5 μM T0 and GW and then LPS (B) or Aβ25–35 (C) as in Fig. 5; IL-6 secretion was measured by ELISA. The values (means ± SEM) indicate the concentration of the cytokines in mg/ml and are normalized to the total cellular protein (TP). LXR treatments are compared to LPS or Aβ25–35 alone by two-tailed Student's t test.*, p < 0.05, **, p < 0.01, and ***, p < 0.001.