QPCR on genomic DNA shows clear difference in ΔC
for the 3 genotypes. A graphical representation of ΔCt between the tubulin and Wld amplicons, for animals of known genotype (N = 36, box and whisker) and animals of unknown genotype (N = 91, scatter). The areas shown in blue represent the 95% confidence limits for each particular genotype as determined from the box and whisker plots. There is a clear trend for each particular genotype.