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Figure 5 | Molecular Neurodegeneration

Figure 5

From: Design of a novel quantitative PCR (QPCR)-based protocol for genotyping mice carrying the neuroprotective Wallerian degeneration slow (Wlds) gene

Figure 5

QPCR can be used to determine copy number in transgenic animals. QPCR carried out on samples of genomic DNA can be used to determine copy number of an insert in rat transgenic lines (Tg) 23 and 79. Although there is some fluctuation in the determined copy number, wild-type (WT) animals are expected to have 2 copies. Heterozygous Wlds(Het-Wld) are expected to have 4 copies and homozygous Wlds(Hom-Wld) should have 6 copies. Analysis of the QPCR data showed the following copy numbers: WT 1.15 ± 0.68 (mean ± SEM) copies (N = 13), Het-Wld 3.87 ± 0.36 copies (N = 7), Hom-Wld 6.10 ± 0.53 copies (N = 7), Tg23 12.34 ± 2.05 (N = 10) and Tg79 19.49 ± 1.52 (N = 14).

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