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Figure 3 | Molecular Neurodegeneration

Figure 3

From: Evidence against roles for phorbol binding protein Munc13-1, ADAM adaptor Eve-1, or vesicle trafficking phosphoproteins Munc18 or NSF as phospho-state-sensitive modulators of phorbol/PKC-activated Alzheimer APP ectodomain shedding

Figure 3

APP metabolism and sAPPα release are not modulated by the phospho-state of Munc18 at serine 306, serine 313, or by dual phosphorylation at both residues. (A) HoloAPP levels (top panel) were determined in the absence (lane 2) or presence of wildtype or phospho-site mutant forms of Munc18 (lanes 4–7) and in the presence of X11 alone (lane 3) or the combination of X11 plus each form of Munc18 (lanes 8–11). (B) Basal and PMA-stimulated sAPPα release were determined in the absence (lanes 1 and 6) or presence of wildtype (lanes 2 and 7) or phospho-site mutant forms of Munc18 (lanes 3–5 and 8–10).

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Molecular Neurodegeneration

ISSN: 1750-1326