Phorbol-stimulated sAPPα secretion is not modulated through Eve-1. HEK293–695 were transiently transfected with either Eve1-b or Eve1-c. Empty vector was used as control (Cont). Cells were treated with PDBu and processed as above. (A) Representative Western blot for sAPPα in the absence (-) or presence (+) of PDBu. (B) Quantification of 3 such experiments.