Figure 2

Immunostaining of 9- and 12-month-old APP and APP/Snca^-/- brains. A. Sections were stained with 6E10 antibody to detect the total number of amyloid beta plaques. Scale bar denotes 100 μm. B. Representation of the number of plaques per section per hemisphere. While the variability was much higher in the APP/Snca^-/- animals, there was no significant difference in the average number of plaques. N = 3 every 4^th section was examined. C. Western Blots of 12-month-old APP and APP/Snca^-/- brain homogenates. The protein was separated using 10% SDS-PAGE, transferred to a nitrocellulose membrane, and probed with antibodies against β-synuclein, synaptophysin and α-tubulin. No differences in protein expression could be detected.