Figure 5

ApoE inhibits EM-mediated lipid release when apoE is associated with EM particles. ApoE-EM complexes were prepared as described in the Experimental Procedures. Neurons were prepared as described in Figure 4 until treatment. Neurons were treated with EM (5 μg/ml); the apoE3-EM complex (10 μg/ml, and 5 μg/ml, respectively) and the apoE4-EM complex (10 μg/ml, and 5 μg/ml, respectively), which was preincubated and isolated by centrifugation; apoE3 (10 μg/ml) and EM (5 μg/ml) or apoE4 (10 μg/ml) and EM (5 μg/ml), which were added separately. Data are means ± S.E. of four samples. *p < 0.05, **p < 0.002, and ***p < 0.02 between the values indicated. Three independent experiments showed similar results.