Figure 2

mir-106a and mir-520c can regulate APP levels post-transcriptionally. APP₇₇₀ and APP₇₅₁ levels are reduced in cells over-expressing (A) mir-106a compared to cells expressing either mir-125b or the empty vector and (B) mir-520c compared to cells expressing the empty vector. (C) Quantification of these Western blot results reveals that mir-106a over-expression significantly decreases APP levels (p < 0.01) compared to cells expressing mir-125b or cells transfected with the empty vector. (D) Quantification of these Western blot results reveals that mir-520c over-expression significantly decreases APP levels (p < 0.01) compared to cells transfected with the empty vector. (E) QPCR results show that APP mRNA levels are not altered by mir-106a or mir-520c over-expression. For all experiments, three independent trials were performed. Error bars represent standard deviation. *p < 0.05; **p < 0.01, compared to the appropriate control.