AIDtg expression in cultured fetal primary neurons increases their sensitivity to toxic and apoptotic stimuli. AIDTg line 59.4.4, at DIV 9. To verify the purity of the neuronal cultures, cells were stained with anti-NeuN (Blue) plus anti-GFAP (red), A, and NeuN (Blue) plus anti-MAP2 (red), B. C Toxicity of indicated stimulus was assessed by measuring LDH release. D Released LDH was weighed against WST-1 uptake in the LDH/WST-1 ratio, which confirms the trend of LDH release. C and D Values from toxic stimuli were weighed against values from untreated cells to express the increase of the indicators of cell damage. Average values refer to at least 3 AIDtg and 3 littermate mice; measurements were done on 6 separate wells of 96 well culture plate for each foetus' neurons. Similar results were obtained for AIDTg 57 line (not shown).