Figure 4

Effects of Dimebon on voltage-gated calcium currents. (A) Representative traces of voltage-gated Ca²⁺ currents evoked by membrane depolarization from -80 mV holding potential to 0 mV in DIV9 WT and YAC128 MSN. The current waveforms recorded in the same cell in the absence of Dimebon (open circle), and in the presence of 1 μM (filled circle), 10 μM (open triangle) and 50 μM (filled diamond) of Dimebon are shown. (B) Dose-dependence of Dimebon block of voltage-gated Ca²⁺ channels. The peak voltage-gated Ca²⁺ currents were normalized to the peak voltage-gated Ca²⁺ currents recorded in the same cell in the absence of Dimebon. The normalized data at each Dimebon concentration were averaged from several experiments and shown as mean ± SE (n = 14 for WT and n = 15 forYAC128 MSN). The IC50 = 50 μM for WT and YAC128 MSN.