Figure 5
From: Evaluation of Dimebon in cellular model of Huntington's disease
Evaluation of Dimebon in in vitro HD assay. Glutamate-induced apoptosis of WT and YAC128 MSN treated with Dimebon at different concentrations. WT and YAC128 MSN at 14 DIV were exposed to 250 μM glutamate for 7 h, fixed, permeabilized and analyzed by TUNEL staining and PI counterstaining. The Dimebon was added 30 min before the application of glutamate. The fraction of TUNEL-positive is plotted against glutamate concentration for WT (open circle) and YAC128 (YAC, filled circles) mice. The data are shown as mean ± SE (n = 6–8 microscopic fields, 100–300 MSN per field). The results in the absence (black symbols) and presence (red symbols) obtained in the presence of 5 μM of Dimebon (A) 10 μM Dimebon (B) and 50 μM Dimebon (C) are compared.