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Figure 3 | Molecular Neurodegeneration

Figure 3

From: Formation of soluble amyloid oligomers and amyloid fibrils by the multifunctional protein vitronectin

Figure 3

Vitronectin forms soluble nonfibrillar amyloid oligomers. Samples were spotted on a nitrocellulose membrane and probed with either A11 (A-D) or vitronectin (E-H) primary antibodies. Recombinant vitronectin (rVn) incubated in 20% HFIP and 1 mM HCl exhibits reactivity to the oligomer-specific A11 antibody (B), whereas vitronectin freshly dissolved in phosphate buffer (A) or treated with 50% HFIP (C) do not, suggesting that treatment with 20% HFIP enriches the population of soluble oligomers. Reactivity of samples with the vitronectin antibody confirms the presence of protein (E-G). Aβ oligomers served as a positive control for the A11 antibody (D), but are not detected by the vitronectin antibody (H).

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Molecular Neurodegeneration

ISSN: 1750-1326