Progressive gene dysregulation in R6/2 mice over time. (A) Raw crossing threshold data for Actb, Grin1 and Ubc in 12 and 15 week old R6/2 transgenic mice and wild-type littermates in striatum shows an increase in crossing threshold data for both Actb and Grin1 but not Ubc, despite carefully controlling for RNA amounts. (B) Similar data as for (A), except that RNA extracts are from cerebellum. 12 week old wild-type (WT) = white bars, 12 week old R6/2 mice = stripes, 15 week old WT = dots, 15 week old R6/2 = solid fill bars. Error bars are S.E.M. (n = 10), * p < 0.05.