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Table 1 Quantitative densitometry of SNCA in situ hybridization

From: In vivo silencing of alpha-synuclein using naked siRNA

  SNCA densitometry R/L ratio   
Brain region PBS (N = 9) Luciferase siRNA (N = 10) SNCA siRNA (N = 11) P-value: SNCA vs. Luciferase P-value: SNCA vs. PBS
Cortex 0.92 (0.82 – 0.98) 0.90 (0.86 – 1.00) 0.27 (0.20 – 0.90) 0.036 0.067
CA1 1.02 (0.98 – 1.14) 0.97 (0.93 – 1.06) 0.35 (0.14 – 0.54) <0.001 <0.001
CA2 1.02 (0.93 – 1.05) 1.01 (0.97 – 1.15) 0.42 (0.19 – 0.62) <0.001 <0.001
CA3 1.15 (0.99 – 1.24) 1.09 (1.01 – 1.15) 0.27 (0.12 – 0.71) <0.001 <0.001
DG 1.02 (0.99 – 1.11) 0.99 (0.94 – 1.04) 0.19 (0.10 – 0.68) 0.008 0.001
  1. Using densitometry to determine efficacy of SNCA siRNA, SNCA expression in the injected (PBS, luciferase siRNA or SNCA siRNA) right side was compared to the uninjected left side and compared across treatment groups. P-values were derived from Wilcoxon rank sum test and the sample median (25th percentile – 75th percentile) is given.