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Table 1 Primary and Secondary Antibodies Used in Western Blots

From: Histopathological and molecular heterogeneity among individuals with dementia associated with Presenilin mutations

Primary Antibody

Antigen Specificity

Secondary Antibody

Company/Catalog #

22C11

AβPP aa. 66–81

M

Millipore/MAB348

CT9APP

Last 9 aa. of AβPP

R

Millipore/AB5352

Notch-1

NICD N-terminal 14 aa.

R

Millipore/AB5709

Erb-B4

C-terminal aa. 1258–1308

R

Santa Cruz/sc-283

N-Cadherin

aa. 802–819

M

BD Transduction Laboratories/610920

Tau

aa. 159–163

M

Pierce/MN1000

Actin Ab-5

Clone C4

M

BD Transduction Laboratories/A65020

Actin

N-terminus of human γ-actin

R

Abcam/Ab37063

  1. AβPP = amyloid-β precursor protein; aa. = amino acid sequence; NICD = Notch-1 intracellular domain; M = goat-anti mouse IgG, HRP conjugated (Pierce); R = goat-anti rabbit IgG, HRP conjugated (Pierce)