Images of JC-1 staining of differentiated SH-SY5Y neural cells exposed to 50 nM rotenone for the indicated number of days. Green corresponds to monomeric JC-1 accumulating in low voltage mitochondria, and red corresponds to J-aggregates that form in highly energized mitochondria. Shown are representative examples of green/red image pairs at different rotenone exposure times. Cells with loss of red JC-1 aggregate staining were not observed at any rotenone exposure time point. Note the absence of neurites in the 21 days neurons.