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Figure 5 | Molecular Neurodegeneration

Figure 5

From: Detergent resistant membrane-associated IDE in brain tissue and cultured cells: Relevance to Aβ and insulin degradation

Figure 5

PM-IDE activity in vitro is impaired after manipulation of membrane lipid composition. A- Representative western blotting of IDE and flotillin-1 showed no differences in the protein levels in membranes isolated from control (CTL) and Mβ CD-treated cells.B- Representative PhosphorImage scan showed degradation of [125I]-insulin after incubation with membranes isolated from cells treated or not with Mβ CD in the presence of a protease inhibitor cocktail (as described in Antibodies and Chemicals) with or without metalloprotease inhibitors (1,10 phenantroline/EDTA). Bars showed the semi-quantitative analysis of the percentage of [125I]-insulin degradation by endogenous IDE from control and MβCD-treated membranes (n = 3; *p < 0.05).

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Molecular Neurodegeneration

ISSN: 1750-1326