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Figure 8 | Molecular Neurodegeneration

Figure 8

From: Detergent resistant membrane-associated IDE in brain tissue and cultured cells: Relevance to Aβ and insulin degradation

Figure 8

Impact of brain cholesterol levels on endogenous IDE localization and activity. A-Sucrose gradient fractions from sel-1 (+/+) and (+/-) mouse brains analyzed by western blotting with anti-IDE and anti-flotillin, respectively. Framed region, DRMs (fractions 3 and 4). Fraction 2: top of the gradient. Fraction 9: bottom of the gradient. B- Upper panel, bars showed the semi-quantitative analysis of the percentage of total endogenous IDE and flotillin-1 immunoreactivity in sucrose gradient fractions 3 and 4 (DRMs) from control (open bars) and partially knocked-out mice (filled bars). IDE and flotillin-1 present in DRM fraction were significant affected in sel-1 (+/-) mice brain compared to (+/+) mice (n = 3; *p < 0.05). C- Bars showed the semi-quantitative analysis of the percentage of [125I]-insulin degradation by endogenous DRMs-bound IDE from sel (+/+) and sel (+/-) brain membranes in the presence of a protease inhibitor cocktail as described in Antibodies and Chemicals (n = 2; *p < 0.05).

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