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Figure 5 | Molecular Neurodegeneration

Figure 5

From: Changes in γ-secretase activity and specificity caused by the introduction of consensus aspartyl protease active motif in Presenilin 1

Figure 5

PS1 carrying D385TG triplet suppresses NICD production. Each indicated stable cell was transfected with the Notch ΔE-GVP, pFR-Luc and pRL-CMV. In Notch ΔE-GVP, the Notch ΔE protein was fused to VP16 transactivating protein domains (GVP). After the cleavage of the N ΔEGVP by the γ-secretase, the Notch ΔE-GVP intracellular domain will translocate to the nucleus and binds to the GAL4 DNA binding domain located in the upstream of the reporter pFR-Luc to activates transcription. The results are the average of the three experiments.

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