Figure 5From: Changes in γ-secretase activity and specificity caused by the introduction of consensus aspartyl protease active motif in Presenilin 1PS1 carrying D385TG triplet suppresses NICD production. Each indicated stable cell was transfected with the Notch ΔE-GVP, pFR-Luc and pRL-CMV. In Notch ΔE-GVP, the Notch ΔE protein was fused to VP16 transactivating protein domains (GVP). After the cleavage of the N ΔEGVP by the γ-secretase, the Notch ΔE-GVP intracellular domain will translocate to the nucleus and binds to the GAL4 DNA binding domain located in the upstream of the reporter pFR-Luc to activates transcription. The results are the average of the three experiments.Back to article page