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Table 3 Transient transfection of various PS1 constructs in HEK-293 cells derived stable cell lines expressing APP carrying Swedish mutation (H125-16).

From: Changes in γ-secretase activity and specificity caused by the introduction of consensus aspartyl protease active motif in Presenilin 1

Cell Line

Transfected PS1 Constructs

Ratio of Aβ42/Aβ

H125.3-16

PS1

0.082 ± 0.022

"

PS1-D385TG

0.261 ± 0.029**

"

PS1-D385TA

0.212 ± 0.007**

"

PS1-D257TA/D385TG

0.186 ± 0.010**

"

PS1- D257TG/D385TA

0.171 ± 0.005**

  1. Aβ40 and Aβ42 levels were measured 48 hrs post-transfection.
  2. ** p < 0.01 (versus PS1 wt) N = 3
  3. Changed residue in the DTG motif was shown in underlined.
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Molecular Neurodegeneration

ISSN: 1750-1326