Changes in γ-secretase activity and specificity caused by the introduction of consensus aspartyl protease active motif in Presenilin 1

Table 4 Effects of selected NSAID compounds on Aβ production from indicated cell lines.

Cell Lines	NSAID	Dose (μM)	Aβ₄₀ (% of vehicle)	Aβ₄₂ (% of vehicle)
APC5 cell line	(S) Ibuprofen	0	100	100
		33	101 ± 8	96 ± 17
		100	96 ± 15	79 ± 21
		300	70 ± 13 *	62 ± 9 *
	Indomethacin	0	100	100
		33	101 ± 11	79 ± 21
		100	93 ± 14	53 ± 12 *
		300	68 ± 11	11 ± 4 *
	Sulindac Sulfide	0	100	100
		11	103	88
		33	96 ± 5	64 ± 2 *
		100	57 ± 2 *	3 ± 1 *
APD3 cell line	(S) Ibuprofen	0	100	100
		33	135 ± 19	88
		100	119 ± 34	64 ± 2 *
		300	103 ± 36	31 ± 11 *
	Indomethacin	0	100	100
		33	95 ± 22	52 ± 15 *
		100	92 ± 12 *	13 ± 11 *
		300	72 ± 16 *	0 *
	Sulindac Sulfide	0	100	100
		11	111	76
		33	128 ± 18	56 ± 4 *
		100	64 ± 10 *	0 *

• * p < 0.05
• Note: at 11 μM Sulindac sulfide only one experiment was run which was the repeat experiment; due to the fact that this drug showed more potency than the others at lower doses that dose was added and the 300 μM dose was omitted since it exhibited toxicity on the cells
Each dose was run in triplicate.

ISSN: 1750-1326