Discrete changes in MBP levels in CNS of H-2b/s and H-2b mice throughout EAE. Discrete changes in MBP levels were observed in spinal cord of H-2b and H-2b/s mice throughout EAE. Relative levels of MBP were measured similarly as levels of MAG (Fig. 1). Compared to control values, modest reduction of MBP levels was measured only in H-2b/s mice during relapsing and chronic-sustained EAE. This antibody revealed presence of four distinct closely related 21–26 KD isoforms of MBP in control and EAE spinal cord, similarly as reported by the manufacturer of this antibody. These isoforms may be the result of posttranslational modification of MBP. Stripped and re-probed membrane was shown. Corresponding levels of GAPDH are shown in Fig. 6.