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Figure 1 | Molecular Neurodegeneration

Figure 1

From: Inducible mutant huntingtin expression in HN10 cells reproduces Huntington's disease-like neuronal dysfunction

Figure 1

Inducible expression of wild-type and mutant huntingtin fragments in clonal HN10 cell lines. A: Scheme of normal (25Q) and mutant (72Q) Htt fragments used for creation of inducible HN10 cell lines. B: Induction of Htt expression with 500 nM RheoSwitch® Ligand (RSL1) normalized for tubulin content in cell lysates demonstrated by western blot analysis. No basal expression is detected in non-induced cells. C: Expression of induced Htt shown by fluorescent immunocytochemistry of Htt cell lines treated in the presence or absence of inducer. Five days of induction with 500 nM RSL1 results in the appearance of Htt inclusions specifically in a subset of 72Q Htt-exon1 cells (white arrows).

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Molecular Neurodegeneration

ISSN: 1750-1326