DJ-1 expression is not essential for proper zebrafish development. A, B. Embryos were injected with control or DJ-1 MO at the one cell stage, and images were acquired at 48 hpf. DJ-1 KD fish did not differ in morphologic phenotype compared to control MO injected zebrafish. C, D. Embryos injected with control or DJ-1 MO were fixed at 48 hpf, followed by in situ hybridization using a probe against TH. TH staining of control or DJ-1 MO injected embryos was almost identical.