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Figure 4 | Molecular Neurodegeneration

Figure 4

From: The PI3K-Akt-mTOR pathway regulates Aβ oligomer induced neuronal cell cycle events

Figure 4

Cell autonomous induction of BrdU and MAP2 process loss in primary neurons from the R1.40 transgenic mouse model of AD. Cultured primary cortical neurons (21 DIV) from non-transgenic (WT, A) and homozygous R1.40 (R/R, B) mouse embryos were incubated with fresh NB media in presence of BrdU for 24 h. Following fixation, cells were immunostained with antibodies against MAP2 (green in A and B) and BrdU (red in A and B) revealing elevated incorporation of BrdU and a decrease in the length of MAP2 positive processes in the R/R cultures. C. Quantification of the percentage of BrdU positive/MAP2 positive cells demonstrated a statistically significant (p = 0.0012; unpaired t test; mean ± SEM; n = 4 independent cultures) increase in R/R cultures when compared to WT cultures. D-F. Quantification of the total dendrite count (total area occupied by all MAP2 positive dendrites per a given field/image) (D) and number of MAP positive processes per cell (E) were not significantly different between WT and R/R cultures, while the number of MAP2 positive processes longer than 5 μm per cell (F) were significantly (p < 0.001; unpaired t test; n = 4 independent cultures) reduced in the R/R cultures when compared to the WT cultures.

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