Aβ increases LRP1 shedding. MEF cells were treated with vehicle control or 1 μM Aβ42 for 48 h. Media and cell lysates were blotted with two LRP1 antibodies to detect either the extracellular LRP1-515 subunit or the transmembrane LRP1-85 subunit. LRP1-515 detected in the media represents sLRP1 and is increased with Aβ42 treatment. Band intensities were analyzed by densitometry from triplicate samples. The ratios of sLRP1 in the media and LRP1-85 in the lysates were compared among three treatments. Note, the relative sLRP1 levels were significantly increased after Aβ42 treatment.