LRP1 shedding is significantly decreased in MEF cells lacking ADAM10 or ADAM17. LRP1 and sLRP1 levels were analyzed by Western blots as in Figure 3. Band intensities were analyzed by densitometry from triplicate samples. The ratios of sLRP1 in the media and LRP1-85 in the lysates were compared among three cell types. Note, the relative sLRP1 levels were significantly decreased in ADAM17-KO and ADAM10-KO MEF cells.