Figure 5
From: LRP1 shedding in human brain: roles of ADAM10 and ADAM17
Over-expression of ADAM10 or ADAM17 increases LRP1 shedding, which is inhibitable by ADAM-specific inhibitor TIMP-3. CHO LRP1-null cells were transiently co-transfected with HA-tagged LRP1 minireceptor mLRP4 together with RAP, ADAM10, ADAM17, and/or TIMP-3 as indicated. The levels of sLRP1 were detected by Western blot using anti-HA antibody.