Figure 2
Amyloid formation in transgenic C. elegans expressing different Aβ variants. A. Images show ThS stained C. elegans that express the Aβ1–42 wt or its variants Arctic and NIC. The visualization of the amyloid deposits (arrow heads) in these strains is shown at 48 h (larval stage L4), 72 h (1 day old adult), 96 h (2 day old adult) and 120 h (3 day old adult) of development. The images are representative of 4 experiments where 40 worms were analyzed for each time point. Bar 20 μm. B. Quantification of amyloid deposit area in ThS stained transgenic C. elegans expressing the Aβ variants mentioned above. The quantification of the amyloid deposit area was also done at 48, 72, 96 and 120 h of development in the head of at least 40 worms for each time point, from the tip of the heat to the end of the pharynx (see Fig. 4A).