Figure 4

STG grey matter mitochondria fraction DJ-1 levels in PD and NPC: A. Super temporal cortex mitochondria were extracted and samples were prepared and analyzed by native and denatured Western blot. B. Spot density analysis of DJ-1 high molecular weight complex and total DJ-1 was normalized to CoxIV level and indicated in percentage to NPC levels. C. The Western blot study demonstrates cell compartments and related proteins in the mitochondria fraction. Specific antibodies that recognize specific cell compartments that are used, anti-CoxIV, anti-prohibitin are for mitochondria, anti-EEA1 is for early endosome antigen1 (EEA1), anti-GM130 is for Golgi Matrix protein (GM130) and anti-LAMP2 is for Lysosome-associated membrane protein-2 (LAMP2). When compare to the brain lysate before purification, CoxIV and prohibitin levels were found to be significantly elevated, more than 10 folds increased based on the densometric analysis, in the purified mitochondria. It shows that though there is little contamination of other cell organelles.