Microphotograph of substantia nigra from controls or PD patients stained for LRRK2. (A, B) control coronal midbrain paraffin section (5 μm) and (C, D) PD midbrain section in 200× (scale bar 50 μm) or 800× (scale bar 20 μm) magnification displaying the substantial loss of cells expressing melanin and LRRK2. The arrows exemplarily show cells expressing both LRRK2 and melanin. (E) Cell counts of melanin and LRRK2 expressing cells in substantia nigra of controls and patients with Parkinson's disease. Note the significant loss of cells expressing melanin and LRRK2 (** P < 0.01, unpaired Student's t-test).