Figure 3From: Emerging role of LRRK2 in human neural progenitor cell cycle progression, survival and differentiationLRRK2 involvement in dopaminergic differentiation of hmNPCs. (A) Representative samples of expanded hmNPCs quantified for LRRK2 or nestin mRNA level as determined 72 h upon nucleofection with either non-specific siRNA (scrambled control siRNA) or LRRK2-specific siRNA, both relative to HMBS mRNA expression. (B) Immunoblot demonstrating knockdown of LRRK2 protein expression in hmNPCs, observed 72 h after siRNA delivery. (C) Densitometric analysis of Western blots exhibiting relative band intensities of unaffected neuronal (Tuj1) and glial (GFAP) markers but reduced TH expression, all normalized to control samples. β-actin served as loading control (D). Quantification of TH+ and TH/caspase-3 double-positive cells expressed as number of immunoreactive cells per visual field (n = 6). Data are means of 3 independent experiments ± S.E.M. *P < 0.05 vs. scrambled siRNA sample.Back to article page