LRRK2 down-regulation in differentiated neuralprogenitors induces cell death. (A) Partial LRRK2 protein reduction induced appearance of dying cells as detected by immunostaining against cleaved caspase-3. Note plenty of red cells expressing activated caspase-3 at lower magnification (A – right panel). Caspase-3 containing cells were not observed in hmNPCs treated with scrambled siRNA before differentiation (A – left panel). (B) Some of the TH+ cells in siLRRK2 cultures were also positive for acivated caspase-3 (higher magnification).