Figure 3
From: Reduced axonal transport in Parkinson's disease cybrid neurites is restored by light therapy
The montage in 3A shows PD61 cybrid neuronal cells stained with the MitoProfile® OXPHOS/PDH immunocytochemistry kit (MitoSciences). Specific antibodies (FITC secondary antibody, green) to Complex I-NDUFB4 (A-C), Complex II-30 kDa (D-F), Complex III-Core 2 protein (G-I), Complex IV-subunit I (J-L), and Complex V-OSCP (M-O) were combined with antibodies to a control subunit (CVα, Texas red secondary, red) to double stain mitochondria in cybrid neuronal cells (see methods). The cell bodies were stained with SYTO 61 (blue). Cybrid neuronal cells in the first column that exhibit double staining are orange or yellow and have intact mETC assembly while cybrid neuronal cells that lack intact assembly of complexs in the mETC are red. Bar = 20 μm. The graph in 3B depicts counts of PD cybrid neuronal cells that contained fully assembled mtETC complexes. In this graph Complex I is black, Complex II has diagonal stripes, Complex III is light gray, Complex IV has horizontal stripes, and Complex V is dark gray. The CNT cybrid neuronal cells exhibited complete assembly of all five complexes (data not shown).