Neurochemical Alterations Caused by αSyn119 Expression in Catecholaminergic Neurons. Analysis of biogenic amines in discrete brain regions of ROSA26-αSyn119 mice by HPLC with electrochemical detection. The level of NE (A) and 5-HT (B) were measured by HPLC in olfactory bulb, cerebral cortex, prefrontal cortex, striatum and brainstem tissues of 10–11 month-old female ROSA26-αSyn119+/+/TH-Cre mice (Cre +) and their ROSA26-αSyn119+/+ littermate controls (Cre -). The concentration of NE and 5-HT is expressed as ng per mg of tissue, and data represent the mean ± SEM (n = 5–7 mice per genotype). Significant differences between Cre - and Cre + genotypes were determined following statistical analysis by unpaired, two-tailed Student's t test (*p < 0.05).